Estradiol (E2) ELISA Kit-ELISA Kit-Products-Abebio

Cat. No.	Size	Price
AE64863GE	48T	260
AE64863GE	96T	480

Technical Data

Species Reactivity

General

UniProt

N/A

Abbreviation

Alternative Names

N/A

Range

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Sensitivity

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Sample Type

Serum, Plasma, Other biological fluids

Detection Method

Competitive

Analysis Method

Quantitive

Assay Duration

1-4.5h

Sample Volume

1-200 μL

Detection Wavelengt

450 nm Test principle:

This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for E2 and Horseradish Peroxidase (HRP) conjugated E2. The competitive inhibition reaction is launched between with HRP labeled E2 and unlabeled E2 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of E2 in the sample. The color development is stopped and the intensity of the color is measured.

Product Overview: Estradiol is an estrogenic hormone, C18H24O2, produced by the ovaries and used in treating estrogen deficiency. Estradiol is essential for maintaining normal female functions and is normally produced by the ovaries. After menopause, the ovaries decrease their production of this hormone. Estradiol is a sex hormone. Estradiol is the predominant sex hormone present in females; however, it is present in males, albeit at lower levels, as well. It represents the major estrogen in humans. Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs including the bones.Estradiol is produced not only in the gonads: In both sexes, precursor hormones, to be specific testosterone, are converted by aromatization to estradiol. In particular, fat cells are active to convert precursors to estradiol, and will continue to do so even after menopause.
Components:

Reagents	Quantity	Reagents	Quantity
Assay plate (96 Wells)	1	Instruction manual	1
Standard	6 x 1 mL	Antibody	1 x 6 mL
HRP-Conjugate	1 x 6 mL	Wash Buffer (concentrate 20 x)	1 x 15 mL
Substrate A	1 x 7 mL	Substrate B	1 x 7 mL
Stop Solution	1 x 7 mL	Adhesive Films	4

Specificity: This assay has high sensitivity and excellent specificity for detection of E2. No significant cross-reactivity or interference between E2 and analogues was observed.
Recovery: Matrices listed below were spiked with certain level of recombinant E2 and the recovery rates were calculated by comparing the measured value to the expected amount of E2 in samples.

Sample Type	Number	Recovery range (%)	Average(%)
Serum	10	90-101	96
EDTA plasma	10	89-97	93
Heparin plasma	10	91-99	95

Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
Linearity: The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample Type	1：2	1：4	1：8	1：16
Serum	78-89%	81-99%	92-103%	95-105%
EDTA plasma	91-101%	90-98%	93-101%	91-98%
Heparin plasma	92-103%	93-102%	92-99%	91-101%

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
Sample collection and storage: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 2 - 8°C before centrifugation for 15 minutes at 1000 × g. Remove serum and assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 × g at 2 - 8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles. Other biological fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Kits storage instructions: Store at 2-8°C. Please refer to Instruction Manual.

Summary

Research topic

Bone and cartilage metabolism

Summary

Vitamin D is mainly synthesized in the skin from 7-dehydrocholesterol and is partially from dietary and supplementation origin. In the liver, Vitamin D is hydroxylated on carbon 25 to produce the intermediate 25OH Vitamin D. 25OH Vitamin D is further metabolized before it can carry out the functions of Vitamin D on intestine, kidneys, bone and other organs and tissues. This subsequent reaction takes place in the kidneys and in other tissues. Thus 25OH Vitamin D is further hydroxylated in the 1α-position to produce 1α,25-dihydroxyvitamin D (1,25(OH)2 Vitamin D). In addition to the above-mentioned tissues, placenta of pregnant women and macrophage cells in case of sarcoidis can also produce some amount of 1,25(OH)2 Vitamin D. 1,25(OH)2 Vitamin D is the active form of Vitamin D with regard to the known functions whereas 25OH Vitamin D and Vitamin D itself can be excluded as being physiologically functional. 1,25(OH)2 Vitamin D stimulates the intestinal absorption of both calcium and phosphorus. It also stimulates bone resorption and mineralization thereby preventing the development of rickets and osteomalacia. 1,25(OH)2 Vitamin D is also be active in other tissues responsible for Calcium transport (placenta, kidney, mammary gland,…) and endocrine glands such as parathyroid glands. 1,25(OH)2 Vitamin D is rapidly metabolized and its halflife is approximately 12h in plasma. Its main metabolite is calcitroic acid, a C-23 carboxylic derivative, essentially without any biological activity. In addition to this pathway, 1,25(OH)2 Vitamin D undergoes 24-hydroxylation to produce 1,24,25-trihydroxyvitamin D. This compound has less biological activity than its parent and this metabolic route is considered as a minor pathway. The levels of 1,25(OH)2 Vitamin D in plasma or serum is 100 to 1000 less than that of 25OH Vitamin D. Due to its low concentrations and the presence of many similar metabolites, the measurement of 1,25(OH)2 Vitamin D requires extraction and separation by chromatography.

Product References (1)

References

Parfieniuk-Kowerda A, Świderska M, Rogalska M, Maciaszek M, Jaroszewicz J,Flisiak R. Chronic hepatitis B virus infection is associated with decreased serum25(OH)D concentration in non-cirrhotic patients. Clin Exp Hepatol. 2019Mar;5(1):75-80. doi: 10.5114/ceh.2019.83160. Epub 2019 Feb 20. PubMed PMID:30915410; PubMed Central PMCID: PMC6431090. See more on PubMed

Summary References (1)

References to 1,25OH2 Vitamin D

Bouillon RA, Auwerx JH, Lissens WD, Pelemans WK. Vitamin D status in the elderly: seasonal substrate deficiency causes 1,25-dihydroxycholecalciferol deficiency. Am J Clin Nutr. 1987 Apr;45 (4):755-63

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Estradiol (E2) ELISA Kit

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