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Recombinant Human Catenin beta-1 (CTNNB1) Protein


Species Reactivity

Human

Source

Prokaryotic expression

Host

E. coli

Synonyms

Catenin beta-1; CTNNB1; CTNNB

Concentration

1 mg/mL

Form

Lyophilized or Liquid

Purity

> 85% as determined by SDS-PAGE

Residues

Ser 29-His 118 (P35222)

Tag

N-terminal His-tag

Purification

Affinity purification

Molecular Weight(MW)

27 kDa

Applications

Western blotting,ELISA;SDS-PAGE;Antigen;

Cat

AP50078HU

Size

100μg 500μg 1mg

Price

Inquire

Availability

3-5 working days

Manual

Inquire
Product Overview:
Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2.
Usage:
Reconstitute in PBS or others.
Stability:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage:
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.
Stroage Buffer:
Lyophilized from 20mM PB, 300mMNaCl,pH7.7
Precautions:
This product is for research use only, not for diagnostic or therapeutic use.