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Recombinant Human Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) Protein


Species Reactivity

Human

Source

Prokaryotic expression

Host

E. coli

Synonyms

BALP; BSAP; HOPS; AP-TNAP; TNSALP; Bone Alkaline Phosphatase; Alkaline Phosphatase,Tissue-Nonspecific Isozyme

Concentration

1 mg/mL

Form

Lyophilized or Liquid

Purity

> 95% as determined by SDS-PAGE

Residues

N/A

Tag

N/A

Purification

Affinity purification

Molecular Weight(MW)

N/A

Applications

Western blotting,ELISA;SDS-PAGE;Antigen;

Cat

AP50062HU

Size

100μg 500μg 1mg

Price

Inquire

Availability

3-5 working days

Manual

Inquire
Product Overview:
Alkaline phosphatase, tissue-nonspecific isozyme is an enzyme encoded by the ALPL gene.There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development.
Usage:
Reconstitute in PBS or others.
Stability:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage:
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.
Stroage Buffer:
The protein solution is in 0.05M phosphate buffer containing 0.15M NaCl and 0.09% NaN3 pH 7.5. Filtered through a 0.25μM membrane
Precautions:
This product is for research use only, not for diagnostic or therapeutic use.