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Recombinant Human Transferrin (TF) Protein


Species Reactivity

Human

Source

Prokaryotic expression

Host

E.coli

Synonyms

TRF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin

Concentration

1 mg/mL

Form

Lyophilized or Liquid

Purity

≥90% by SDS-PAGE

Residues

Val347~Lys698 (P02787)

Tag

N-terminal His-tag

Purification

Affinity purification

Molecular Weight(MW)

45 kDa

Applications

Western blotting,ELISA;SDS-PAGE;Antigen;

Cat

AP50218HU

Size

100μg 500μg 1mg

Price

Inquire

Availability

3-4 weeks

Manual

Inquire
Product Overview:
Transferrin is a blood plasma protein for iron ion delivery that, in humans, is encoded by the TF gene. Transferrin is a glycoprotein that binds iron very tightly but reversibly. Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h). Transferrin has a molecular weight of around 80 kiloDaltons and contains 2 specific high-affinity Fe(III) binding sites.
The affinity of transferrin for Fe(III) is extremely high (1023 M-1 at pH 7.4)[citation needed] but decreases progressively with decreasing pH below neutrality.In humans, transferrin consists of a poly peptide chain containing 679 amino acids. It is a complex composed of alpha helices and beta sheets to form two domains.

Usage:
Reconstitute in PBS or others.
Stability:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage:
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.

Stroage Buffer:
Protein lyophilized in sterile PBS (58 mM Na2HPO4, 17 mM NaH2PO4, 68 mM NaCl, 300 mM Imidazole, pH 8.0). Trehalose (5-8%) and 10% glycerol.

Precautions:
This product is for research use only, not for diagnostic or therapeutic use.