Home // Products // ELISA Kit

Sheep Pancreas/duodenum homeobox protein 1 (PDX1) ELISA Kit


Species Reactivity

Sheep (Ovis aries)

UniProt

Abbreviation

PDX1

Alternative Names

GSF; IDX-1; IPF1; IUF1; MODY4; PDX-1; STF-1; glucose-sensitive factor|insulin promoter factor 1; homeodomain transcription factor|insulin upstream factor 1|pancreatic-duodenal homeobox factor 1|soma

Range

Request Information

Sensitivity

Request Information

Sample Type

Serum, Plasma, Other biological fluids

Detection Method

Sandwich

Analysis Method

Quantitive

Assay Duration

1-4.5h

Sample Volume

1-200 μL

Detection Wavelengt

450 nm

Cat

AE64478SH

Size

96T 48T 96T*5 96T*50

Price

Inquire

Availability

3-5 working days

Manual

Inquire
Test principle:
This assay employs a two-site sandwich ELISA to quantitate PDX1 in samples. An antibody specific for PDX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:
PDX1 (pancreatic and duodenal homeobox 1), also known as insulin promoter factor 1, is a transcription factor necessary for pancreatic development, including 
Components:

Reagents

Quantity

Reagents

Quantity

Assay plate (96 Wells)

1

Instruction manual

1

Standard (lyophilized)

2

Sample Diluent

1 x 20 mL

Biotin-Conjugate (concentrate 100 x)

1 x 120 μL

Biotin-Conjugate Diluent

1 x 20 mL

Streptavidin-HRP  (concentrate 100 x)

1 x 120 μL

Streptavidin-HRP Diluent   

1 x 20 mL

Wash Buffer (concentrate 25 x)

1 x 20 mL

Substrate Solution

1 x 12 mL

Stop Solution

1 x 10 mL

Adhesive Films

4

Specificity:

Recovery:

Sample Type

Number

Recovery range (%)

Average(%)

Serum

10

90-101

96

EDTA plasma

10

89-97

93

Heparin plasma

10

91-99

95

Precision:

Linearity:

Sample Type

1:2

1:4

1:8

1:16

Serum

78-89%

81-99%

92-103%

95-105%

EDTA plasma

91-101%

90-98%

93-101%

91-98%

Heparin plasma

92-103%

93-102%

92-99%

91-101%

Stability:

Sample collection and storage:

Kits storage instructions: