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Goat Peste des Petits Ruminants Virus antibody (PPRV-Ab) ELISA Kit


Species Reactivity

Goat (Capra hircus; Caprine)

UniProt

N/A

Abbreviation

PPRV-Ab

Alternative Names

N/A

Range

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Sensitivity

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Sample Type

Serum, Plasma, Other biological fluids

Detection Method

Indirect ELISA

Analysis Method

Qualitative

Assay Duration

1-3h

Sample Volume

1-200 μL

Detection Wavelengt

450 nm

Cat

AE64486GO

Size

96T 48T

Price

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Availability

3-5 working days

Manual

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Test principle:
The microtiter plate provided in this kit has been pre-coated with PPRV-Ag. Samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated Anti-Goat PPRV-Ab and incubated. Then substrate solutions are added to each well. Only those wells that contain PPRV-Ab and enzyme-conjugated Anti-Goat PPRV-Ab will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. 

Product Overview:
Ovine rinderpest, also commonly known as peste des petits ruminants (PPR), is a contagious disease primarily affecting mainly goats and sheep; however, camels and wild small ruminants can also be affected. PPR is currently present in North, Central, West and Eastern Africa, the Middle East, South Asia. It is caused by Small ruminants morbillivirus in the genus Morbillivirus, and is closely related to, among others, rinderpest morbillivirus, measles morbillivirus and canine morbillivirus (previously known as canine distemper virus). The disease is highly contagious, and can have a 80-100% mortality rate in acute cases in an epidemic setting. This virus does not infect humans.

Components:

Reagents

Quantity

Reagents

Quantity

Assay plate (96 Wells)

1

Instruction manual

1

Standard (lyophilized)

2

Sample Diluent

1 x 20 mL

Biotin-Conjugate (concentrate 100 x)

1 x 120 μL

Biotin-Conjugate Diluent

1 x 20 mL

Streptavidin-HRP  (concentrate 100 x)

1 x 120 μL

Streptavidin-HRP Diluent   

1 x 20 mL

Wash Buffer (concentrate 25 x)

1 x 20 mL

Substrate Solution

1 x 12 mL

Stop Solution

1 x 10 mL

Adhesive Films

4

Specificity:
This assay has high sensitivity and excellent specificity for detection of Goat PPRV-Ab. No significant cross-reactivity or interference between Goat PPRV-Ab and analogues was observed.
Recovery:
Matrices listed below were spiked with certain level of recombinant Goat PPRV-Ab and the recovery rates were calculated by comparing the measured value to the expected amount of Goat PPRV-Ab in samples.

Sample Type

Number

Recovery range (%)

Average(%)

Serum

10

90-101

96

EDTA plasma

10

89-97

93

Heparin plasma

10

91-99

95

Precision:
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Goat PPRV-Ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample Type

1:2

1:4

1:8

1:16

Serum

78-89%

81-99%

92-103%

95-105%

EDTA plasma

91-101%

90-98%

93-101%

91-98%

Heparin plasma

92-103%

93-102%

92-99%

91-101%

Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
Sample collection and storage:
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 2 - 8°C before centrifugation for 15 minutes at 1000 × g. Remove serum and assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 × g at 2 - 8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles. Other biological fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Kits storage instructions:
Store at 2-8°C. Please refer to Instruction Manual.