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Rabbit Anti-Human Dok-2 Polyclonal Antibody


Species Reactivity

Human

Source

Polyclonal antibody preparation

Host

Rabbit

Synonyms

DOK2; Docking protein 2; Downstream of tyrosine kinase 2; p56(dok-2)

Concentration

1 mg/mL

Form

Lyophilized or Liquid

Purity

> 90% as determined by SDS-PAGE

Molecular Mass

46 kDa

Ig Type

IgG

Purification

Rabbit Polyclonal IgG, the antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Label

None

Immunogen

The antiserum was produced against synthesized peptide derived from human Dok-2. AA range: 266-315 (Q60496).

Applications

WB; IHC; IF; ELISA

Cat

PA84257HU

Size

100μg 500μg

Price

Inquire

Availability

3-5 working days

Manual

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Product Overview:
Docking protein 2 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells.The DOK2 cDNA clone encodes a 412-amino acid protein with a molecular mass of 53 to 56 kD. The DOK2 protein has a potential pleckstrin homology (PH) domain at the N terminus, 13 potential tyrosine phosphorylation sites, 6 PXXP motifs, and 2 YXXPXD motifs (predicted Ras-GAP SH2 domain binding sites). It shares 34.8% overall amino acid identity with another tyrosine-phosphorylated protein, p62dok (DOK1), which also contains a predicted PH domain at its N terminus.

Specifity:
Dok-2 Polyclonal Antibody detects endogenous levels of Dok-2 protein.
Usage:
WB 1:500-2000; IHC 1:100-300; IF 1:200-1000; ELISA 1:20000; Not yet tested in other applications.
Stability:
Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.

Storage:
Shipped at 2-8°C, Store it under sterile conditions at -20°C to -80°C upon receiving. **Avoid repeated freeze-thaw cycles.**

Stroage Buffer:
0.01M PBS (pH 7.4) with 0.5% BSA, 50% glycerol and 0.02% sodium azide.
Precautions:
This product is for research use only, not for diagnostic or therapeutic use.