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Rabbit Anti-Human Dok-4 Polyclonal Antibody


Species Reactivity

Human

Source

Polyclonal antibody preparation

Host

Rabbit

Synonyms

DOK4; Docking protein 4; Downstream of tyrosine kinase 4; Insulin receptor substrate 5; IRS-5; IRS5

Concentration

1 mg/mL

Form

Lyophilized or Liquid

Purity

> 90% as determined by SDS-PAGE

Molecular Mass

37 kDa

Ig Type

IgG

Purification

Rabbit Polyclonal IgG, the antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Label

None

Immunogen

The antiserum was produced against synthesized peptide derived from human Dok-4. AA range: 11-60 (Q8TEW6).

Applications

WB; IHC; IF; ELISA

Cat

PA85947HU

Size

100μg 500μg

Price

Inquire

Availability

3-5 working days

Manual

Inquire
Product Overview:
DOK4 is rapidly and heavily phosphorylated in response to insulin and, once phosphorylated, binds a set of SH2 domain proteins, including RasGAP, CRK, and the nonreceptor tyrosine kinases SRS and FYN. DOK4 also activates MAPK in cells. In contrast, DOK5 is phosphorylated much more slowly and does not associate with this set of SH2 homology domains or activate MAPK. In addition to the PH and PTB domains, DOK4 contains a short C terminus with 5 potential sites for tyrosine phosphorylation. By RT-PCR analysis, Favre et al. (2003) detected expression of DOK4 and DOK5 in peripheral blood T cells. They noted that DOK5 is expressed in activated but not resting T cells, suggesting that the expression of DOK5 is regulated upon T cell activation.

Specifity:
Dok-4 Polyclonal Antibody detects endogenous levels of Dok-4 protein.
Usage:
ELISA=1:5000-20000; WB=1:500-2000; IHC=1:100-300; IF=1:200-1000; Not yet tested in other applications.
Stability:
Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.
Storage:
Shipped at 2-8°C, Store it under sterile conditions at -20°C to -80°C upon receiving. **Avoid repeated freeze-thaw cycles.**

Stroage Buffer:
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Precautions:
This product is for research use only, not for diagnostic or therapeutic use.