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Recombinant Human Fibroblast growth factor 2 (FGF2) Protein


Species Reactivity

Human (Homo sapiens)

Source

Prokaryotic expression

Host

E. coli

Synonyms

B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2

Concentration

1 mg/mL

Form

Liquid

Purity

> 85% as determined by SDS-PAGE

Residues

143-288aa (P09038)

Tag

EP, N-terminal 6xHis-tagged

Purification

Affinity purification

Molecular Weight(MW)

20.4 KD

Applications

Western blotting,ELISA;SDS-PAGE;Antigen;

Cat

AP50258HU

Size

100μg 500μg 1mg

Price

Inquire

Availability

3-5 weeks

Manual

Inquire
Product Overview:
FGF basic is one of at least 22 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation.Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely. The predicted 18 kDa FGF basic isoform can be located in both the cytoplasm and the nucleus and is presumed to be the form secreted. Transcription from alternate start sites produces 21-23 kDa forms found only in the nucleus. High and low molecular weight human FGF basic targets the expression of different genes when expressed in NIH-3T3 cells.The 18 kDa rat sequence has 98% aa identity with mouse, and 96 - 97% identity with human, bovine and sheep FGF basic.

Usage:
Reconstitute in PBS or others.
Stability:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage:
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.

Stroage Buffer:
Tris-based buffer, 50% glycerol
Precautions:
This product is for research use only, not for diagnostic or therapeutic use.