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Dimetridazole ELISA Kit


Species Reactivity

N/A

UniProt

N/A

Abbreviation

Dimetridazole

Alternative Names

N/A

Range

0.01-0.81 ppb

Sensitivity

0.01 ppb

Sample Type

tissue, honey, raw milk, raw egg, cooked egg

Detection Method

Competitive ELISA

Analysis Method

Quantitive

Assay Duration

1-3h

Sample Volume

10-200 μL

Detection Wavelengt

450 nm

Cat

AE81076SF

Size

48T 96T

Price

Inquire

Availability

3-5 working days

Manual

Inquire
Test principle:
This assay is based on the competitive enzyme immunoassay for the detection of Dimetridazole in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Dimetridazole in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Dimetridazole antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Dimetridazole in it. This value is compared to the standard curve and the Dimetridazole concentration is subsequently obtained.
Product Overview:
Dimetridazole is a nitroimidazole class drug that combats protozoan infections. Target: Antiparasitic Dimetridazole (DMZ) is a 5-nitroimidazole drug traditionally used for the prevention and treatment of histomoniasis in turkeys, trichomoniasis in pigeons, genital trichomoniasis in cattle and the prevention and treatment of haemorrhagic enteritis in pigs.

Components:

Reagents

Quantity

Reagents

Quantity

Assay plate (96 Wells)

1

Instruction manual

1

Standard (lyophilized)

2

Sample Diluent

1 x 20 mL

Biotin-Conjugate (concentrate 100 x)

1 x 120 μL

Biotin-Conjugate Diluent

1 x 20 mL

Streptavidin-HRP  (concentrate 100 x)

1 x 120 μL

Streptavidin-HRP Diluent   

1 x 20 mL

Wash Buffer (concentrate 25 x)

1 x 20 mL

Substrate Solution

1 x 12 mL

Stop Solution

1 x 10 mL

Adhesive Films

4

Specificity:

Recovery:

Sample Type

Number

Recovery range (%)

Average(%)

Serum

10

90-101

96

EDTA plasma

10

89-97

93

Heparin plasma

10

91-99

95

Precision:
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
Linearity:

Sample Type

1:2

1:4

1:8

1:16

Serum

78-89%

81-99%

92-103%

95-105%

EDTA plasma

91-101%

90-98%

93-101%

91-98%

Heparin plasma

92-103%

93-102%

92-99%

91-101%

Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
Sample collection and storage:
Store at 2-8°C. Please refer to Instruction Manual.
Kits storage instructions:
Store at 2-8°C. Please refer to Instruction Manual.