Clenbuterol (CLE) ELISA Kit

This assay is based on the competitive enzyme immunoassay for the detection of CLE in the sample. The coupling antigen is pre-coated on the micro-well stripes. The CLE in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-CLE antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the CLE in the sample. This value is compared to the standard curve and the CLE residues is subsequently obtained.
 

Clenbuterol, marketed as Spiropent and Ventipulmin, is a sympathomimetic amine used by sufferers of breathing disorders as a decongestant and bronchodilator. People with chronic breathing disorders such as asthma use this as a bronchodilator to make breathing easier. It is most commonly available as the hydrochloride salt, clenbuterol hydrochloride. Clenbuterol is a 
 

Reagents	Quantity	Reagents	Quantity
Assay plate (96 Wells)	1	Instruction manual	1
Standard	6 x 1 mL	Redissolving Solution (concentrate 10 x)	2 x 20 mL
Antibody	1 x 6 mL	HRP-Conjugate	1 x 6 mL
Wash Buffer (concentrate 20 x)	1 x 20 mL	Stop Solution	1 x 6 mL
Substrate A	1 x 6 mL	Substrate B	1 x 6 mL
Adhesive Films	4

 

 

Sample Type	Number	Recovery range (%)	Average(%)
Serum	10	90-101	96
EDTA plasma	10	89-97	93
Heparin plasma	10	91-99	95

 

 

Sample Type	1：2	1：4	1：8	1：16
Serum	78-89%	81-99%	92-103%	95-105%
EDTA plasma	91-101%	90-98%	93-101%	91-98%
Heparin plasma	92-103%	93-102%	92-99%	91-101%