Salbutamol (SAL) ELISA Kit

This assay is based on the competitive enzyme immunoassay for the detection of SAL in the sample. The coupling antigen is pre-coated on the micro-well stripes. The SAL in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-SAL antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the SAL in the sample. This value is compared to the standard curve and the SAL residues is subsequently obtained.
 

Salbutamol, also known as albuterol and marketed as Ventolin among other names, is a medication that opens up the medium and large airways in the lungs. It is used to treat asthma, exercise-induced bronchospasm, and chronic obstructive pulmonary disease (COPD). It may also be used to treat high blood potassium levels. It is usually used by inhaler or nebulizer but is also available as a pill and intravenous solution. Onset of action of the inhaled version is typically within 15 minutes and lasts for two to six hours. Common side effects include shakiness, headache, fast heart rate, dizziness, and feeling anxious. Serious side effects may include worsening bronchospasm, irregular heartbeat, and low blood potassium levels. It can be used during pregnancy and breastfeeding, but safety is not entirely clear. Salbutamol is a short-acting 
 

Reagents	Quantity	Reagents	Quantity
Assay plate (96 Wells)	1	Instruction manual	1
Standard	6 x 1 mL	Redissolving Solution (concentrate 10 x)	1 x 20 mL
Antibody	1 x 6 mL	HRP-Conjugate	1 x 6 mL
Wash Buffer (concentrate 20 x)	1 x 20 mL	Stop Solution	1 x 6 mL
Substrate A	1 x 6 mL	Substrate B	1 x 6 mL
Adhesive Films	4

 

 

Sample Type	Number	Recovery range (%)	Average(%)
Serum	10	90-101	96
EDTA plasma	10	89-97	93
Heparin plasma	10	91-99	95

 

 

Sample Type	1：2	1：4	1：8	1：16
Serum	78-89%	81-99%	92-103%	95-105%
EDTA plasma	91-101%	90-98%	93-101%	91-98%
Heparin plasma	92-103%	93-102%	92-99%	91-101%