Florfenicol (FF) ELISA Kit

This assay is based on the competitive enzyme immunoassay for the detection of FF in the sample. The coupling antigens are pre-coated on the micro-well stripes. The FF in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-FF antibodies. After the addition of the HRP-conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the FF in it. This value is compared to the standard curve and the FF concentration is subsequently obtained.
 

Florfenicol
 

Reagents	Quantity	Reagents	Quantity
Assay plate (96 Wells)	1	Instruction manual	1
Standard	6 x 1 mL	Sample Diluent (concentrate 2 x)	2 x 20 mL
Antibody	1 x 7 mL	HRP-Conjugate	1 x 12 mL
Wash Buffer (concentrate 20 x)	2 x 20 mL	Stop Solution	1 x 7 mL
Substrate A	1 x 7 mL	Substrate B	1 x 7 mL
Adhesive Films	4

 

 

Sample Type	Number	Recovery range (%)	Average(%)
Serum	10	90-101	96
EDTA plasma	10	89-97	93
Heparin plasma	10	91-99	95

 

 

Sample Type	1：2	1：4	1：8	1：16
Serum	78-89%	81-99%	92-103%	95-105%
EDTA plasma	91-101%	90-98%	93-101%	91-98%
Heparin plasma	92-103%	93-102%	92-99%	91-101%