Product Details
Species Reactivity |
General |
UniProt |
N/A |
Abbreviation |
AFB1 |
Alternative Names |
N/A |
Range |
0.03-0.48 ppb |
Sensitivity |
0.03 ppb |
Sample Type |
cereal, compound feed, cooking oil, peanut, sauce, wheat and other feed, beer, wine, soy sauce, vinegar, etc. |
Detection Method |
Competitive ELISA |
Analysis Method |
Quantitive |
Assay Duration |
1-3h |
Sample Volume |
1-200 μL |
Detection Wavelengt |
450 nm |
Test principle
This assay is based on the competitive enzyme immunoassay for the detection of AFB1 in the sample. The coupling antigens are pre-coated on the micro-well stripes. The AFB1 in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AFB1 antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the AFB1 in it. This value is compared to the standard curve and the AFB1 concentration is subsequently obtained.
Product Overview
Aflatoxin B1 is an aflatoxin produced by Aspergillus flavus and A. parasiticus. It is arguably the most potent carcinogen known, and is up to twice as carcinogenic as an equitoxic dose of X-rays.
Aflatoxin B1 can permeate through the skin. Dermal exposure to this aflatoxin in particular environmental conditions can lead to major health risks.
Aflatoxin B1 is derived from both a dedicated fatty acid synthase (FAS) and a polyketide synthase (PKS), together known as norsolorinic acid synthase. The biosynthesis begins with the synthesis of hexanoate by the FAS, which then becomes the starter unit for the iterative type I PKS. The PKS adds seven malonyl-CoA extenders to the hexanoate to form the C20 polyketide compound. The PKS folds the polyketide in a particular way to induce cyclization to form the anthraquinone norsolorinic acid. A reductase then catalyzes the reduction of the ketone on the norsolorinic acid side-chain to yield averantin. Averantin is converted to averufin via a two different enzymes, a hydroxylase and an alcohol dehydrogenase. This will oxygenate and cyclize averantin's side chain to form the ketal in averufin.
Components
Reagents |
Quantity |
Reagents |
Quantity |
Assay plate (96 Wells) |
1 |
Instruction manual |
1 |
Standards |
6 x 1 mL |
Redissolving Solution (concentrate 2 x) |
2 x 20 mL |
Antibody |
1 x 6 mL |
HRP-Conjugate |
1 x 6 mL |
Wash Buffer (concentrate 20 x) |
1 x 20 mL |
Stop Solution |
1 x 6 mL |
Substrate A |
1 x 6 mL |
Substrate B |
1 x 6 mL |
Adhesive Films |
4 |
|
|
Recovery
Sample Type |
Number |
Recovery range (%) |
Average(%) |
Serum |
10 |
90-101 |
96 |
EDTA plasma |
10 |
89-97 |
93 |
Heparin plasma |
10 |
91-99 |
95 |
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV<8%
Inter-Assay: CV<12%
Linearity
Sample Type |
1:2 |
1:4 |
1:8 |
1:16 |
Serum |
78-89% |
81-99% |
92-103% |
95-105% |
EDTA plasma |
91-101% |
90-98% |
93-101% |
91-98% |
Heparin plasma |
92-103% |
93-102% |
92-99% |
91-101% |
Stability
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
Sample collection and storage
Store at 2-8°C. Please refer to Instruction Manual.
Kits storage instructions
Store at 2-8°C. Please refer to Instruction Manual.