Dexamethasone (DEX) ELISA Kit

This assay is based on the competitive enzyme immunoassay for the detection of DEX in the sample. The coupling antigens are pre-coated on the micro-well stripes. The DEX in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-DEX antibodies. After the addition of the HRP-Conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the DEX in it. This value is compared to the standard curve and the DEX concentration is subsequently obtained.  

Dexamethasone is a potent synthetic member of the glucocorticoid class of steroid drugs that has anti-inflammatory and immunosuppressant effects. It is 25 times more potent than cortisol in its glucocorticoid effect, while having minimal mineralocorticoid effect. Dexamethasone is used to treat many inflammatory and autoimmune conditions, such as rheumatoid arthritis and bronchospasm. Idiopathic thrombocytopenic purpura, decreased numbers of platelets due to an immune problem, responds to 40 mg daily for four days; it may be administered in 14-day cycles. To synthesize dexamethasone, 16β-methylprednisolone acetate is dehydrated to the 9,11-dehydro derivative. This is then reacted with a source of hypobromite, such as basic N-bromosuccinimide, to form the 9α-bromo-11β-hydrin derivative, which is then ring-closed to an epoxide. A ring-opening reaction with hydrogen fluoride in tetrahydrofuran gives dexamethasone.