Rabbit anti-human Insulin (INS) PolyClonal antibody

Insulin is a polypeptide hormone originating in the beta cells of the pancreas and serving as a principal regulator for the storage and production of carbohydrates. Its secretion is normally stimulated by increases in the amount of glucose in circulation. This leads to higher insulin levels and more rapid tissueassimilation of glucose followed by a decline in the insulin level as the glucose level subsides. In a number of conditions, notably insulinoma and diabetes, this relationship is impaired. Insulin tends to circulate at inappropriately high levels in patients with insulin-secreting pancreatic tumors; such tumors can thus be a cause of hypoglycemia. Accordingly, insulin immunoassays used sometimes in connection with provocative doses of tolbutamide or calcium play an essential role in the identification (and localization) of insulinomas.
 

The antibody is a Rabbit polyclonal antibody raised against INS. It has been selected for its ability to recognize INS in ELISA Kit and western blotting.
 

WB=1:100-500 ELISA=1:500-1000 Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
 

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.
 

Shipped at 2-8°C. Upon delivery aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
 

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
 

This product is for research use only, not for diagnostic or therapeutic use.