Product Details
| Species Reactivity |
Human |
| Source |
Prokaryotic expression |
| Host |
E. coli |
| Synonyms |
ALP; ALKP; ALPase; Alk Phos |
| Concentration |
1 mg/mL |
| Form |
Lyophilized or Liquid |
| Purity |
> 95% as determined by SDS-PAGE |
| Residues |
B2R6Q2 |
| Tag |
N-terminal His-tag |
| Purification |
Affinity purification |
| Molecular Weight(MW) |
15 kDa |
| Applications |
Western blotting,ELISA;SDS-PAGE;Antigen; |
Product Overview
Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.
Usage
Reconstitute in PBS or others.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.
Stroage Buffer
Lyophilized from 50mM Tris-HCL，0.5mM EDTA，50mM NaCl，pH 7.4
Precautions
This product is for research use only, not for diagnostic or therapeutic use.
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