Product Details
Species Reactivity |
Human |
Source |
Prokaryotic expression |
Host |
E. coli |
Synonyms |
PRKCA; AAG6; PKC-alpha; PKCA; PRKACA |
Concentration |
1 mg/mL |
Form |
Lyophilized or Liquid |
Purity |
> 90% as determined by SDS-PAGE |
Residues |
Lys562-Val672 (P17252) |
Tag |
N-terminal His-tag |
Purification |
Affinity purification |
Molecular Weight(MW) |
13 kDa |
Applications |
Western blotting,ELISA;SDS-PAGE;Antigen; |
Product Overview
Protein kinase C alpha (PKCα) is an enzyme that in humans is encoded by the PRKCA gene.
Protein kinase C-alpha (PKC-α) is a specific member of the protein kinase family. These enzymes are characterized by their ability to add a phosphate group to other proteins, thus changing their function. PKC-α has been widely studied in the tissues of many organisms including drosophila, xenopus, cow, dog, chicken, human, monkey, mouse, pig, and rabbit. Many studies are currently being conducted investigating the structure, function, and regulation of this enzyme. The most recent investigations concerning this enzyme include its general regulation, hepatic function, and cardiac function.
Usage
Reconstitute in PBS or others.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.
Stroage Buffer
Lyophilized from 50mM Tris-HCL,0.5mM EDTA,300mM NaCl,pH 9.0
Precautions
This product is for research use only, not for diagnostic or therapeutic use.