Product Details
Species Reactivity |
Human |
Source |
Prokaryotic expression |
Host |
E. coli |
Synonyms |
CSF1; CSF-1; MCSF |
Concentration |
1 mg/mL |
Form |
Lyophilized or Liquid |
Purity |
> 95% as determined by SDS-PAGE |
Residues |
Glu33- Lys132 (P09603) |
Tag |
N-terminal His-tag |
Purification |
Affinity purification |
Molecular Weight(MW) |
13 kDa |
Applications |
Western blotting,ELISA;SDS-PAGE;Antigen; |
Product Overview
The colony stimulating factor 1 (CSF1), also known as macrophage colony-stimulating factor (M-CSF), is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. Eukaryotic cells also produce M-CSF in order to combat intercellular viral infection. (See colony-stimulating factor.) M-CSF binds to the Colony stimulating factor 1 receptor. It may also be involved in development of the placenta.
M-CSF is a cytokine. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors.
Four transcript variants encoding three different isoforms have been found for this gene.
Usage
Reconstitute in PBS or others.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.
Stroage Buffer
Lyophilized from 50mM Tris-cl,0.5mM EDTA ,300mM NaCl,pH 9.0
Precautions
This product is for research use only, not for diagnostic or therapeutic use.