Product Details
Species Reactivity |
Human |
Source |
Prokaryotic expression |
Host |
E. coli |
Synonyms |
ERBA2L; PDI; PDIA1; PO4DB |
Concentration |
1 mg/mL |
Form |
Lyophilized or Liquid |
Purity |
> 95% as determined by SDS-PAGE |
Residues |
Gln 93-Gln 197 (P07237) |
Tag |
N-terminal His-tag |
Purification |
Affinity purification |
Molecular Weight(MW) |
12 kDa |
Applications |
Western blotting,ELISA;SDS-PAGE;Antigen; |
Product Overview
This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP
Usage
Reconstitute in PBS or others.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.
Stroage Buffer
Lyophilized from 20mM PB, 300mMNaCl,pH7.7
Precautions
This product is for research use only, not for diagnostic or therapeutic use.