Product Details
Species Reactivity |
Human |
Source |
Prokaryotic expression |
Host |
E. coli |
Synonyms |
CSTA; AREI; STF1; STFA |
Concentration |
1 mg/mL |
Form |
Lyophilized or Liquid |
Purity |
> 95% as determined by SDS-PAGE |
Residues |
Full length protein (P01040) |
Tag |
N-terminal His-tag |
Purification |
Affinity purification |
Molecular Weight(MW) |
12 kDa |
Applications |
Western blotting,ELISA;SDS-PAGE;Antigen; |
Product Overview
Cystatin-A is a protein that in humans is encoded by the CSTA gene.
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer.
Usage
Reconstitute in PBS or others.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.
Stroage Buffer
Lyophilized from 20 mM PB, 300 mM NaCl,pH 7.4
Precautions
This product is for research use only, not for diagnostic or therapeutic use.