Product Details
Species Reactivity |
Human |
Source |
Prokaryotic expression |
Host |
E. coli |
Synonyms |
GAL; GAL-GMAP; GALN; GLNN; GMAP |
Concentration |
1 mg/mL |
Form |
Lyophilized or Liquid |
Purity |
> 90% as determined by SDS-PAGE |
Residues |
Ala20~Ser123 (P22466) |
Tag |
N-terminal His-tag |
Purification |
Affinity purification |
Molecular Weight(MW) |
12 kDa |
Applications |
Western blotting,ELISA;SDS-PAGE;Antigen; |
Product Overview
Galanin is a neuropeptide encoded by the GAL gene, that is widely expressed in the brain, spinal cord, and gut of humans as well as other mammals. Galanin signaling occurs through three G protein-coupled receptors.
The functional role of galanin remains largely unknown; however, galanin is predominately involved in the modulation and inhibition of action potentials in neurons. Galanin has been implicated in many biologically diverse functions, including: nociception, waking and sleep regulation, cognition, feeding, regulation of mood, regulation of blood pressure, it also has roles in development as well as acting as a trophic factor. Galanin neurons in the medial preoptic area of the hypothalamus may govern parental behaviour. Galanin is linked to a number of diseases including Alzheimer's disease, epilepsy as well as depression, eating disorders and cancer. Galanin appears to have neuroprotective activity as its biosynthesis is increased 2-10 fold upon axotomy in the peripheral nervous system as well as when seizure activity occurs in the brain. It may also promote neurogenesis.
Usage
Reconstitute in PBS or others.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.
Stroage Buffer
Lyophilized from 20 mM PB, 300 mM NaCl,pH 7.4
Precautions
This product is for research use only, not for diagnostic or therapeutic use.