Product Details
Species Reactivity |
Human |
Source |
Prokaryotic expression |
Host |
E. coli |
Synonyms |
TSH-B |
Concentration |
1 mg/mL |
Form |
Lyophilized or Liquid |
Purity |
> 95% as determined by SDS-PAGE |
Residues |
N/A |
Tag |
N/A |
Purification |
Affinity purification |
Molecular Weight(MW) |
N/A |
Applications |
Western blotting,ELISA;SDS-PAGE;Antigen; |
Product Overview
Thyrotropin-stimulating hormone (TSH) is a noncovalently linked glycoprotein heterodimer and is part of a family of pituitary hormones containing a common alpha subunit (TSHA) and a unique beta subunit that confers specificity.Using bovine TSHB cDNA as probe, Hayashizaki et al. (1985) cloned TSHB from human liver and leukocyte genomic DNA libraries. Human TSHB encodes a deduced protein consisting of a 20-amino acid signal sequence, a mature protein of 112 amino acids, and a C-terminal extension of 6 hydrophobic amino acids. Wondisford et al. (1988) also cloned the human TSHB gene.Wondisford et al. (1988) determined that the TSHB gene contains 3 exons, the first of which is noncoding. The rat TSHB gene also contains one 5-prime noncoding exon, whereas the mouse Tshb gene contains 3.
Usage
Reconstitute in PBS or others.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.
Stroage Buffer
Lyophilized from sterile 20mM PB,240mM NaCl,PH6.32.
Precautions
This product is for research use only, not for diagnostic or therapeutic use.