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Ofloxacin (OFX) ELISA Kit

Citations(0)Uniprot : N/A
  • Cat.No.: AE81071SF

  • Reactivity: N/A

To Purchase AE81071SF

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48T 96T
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Product Details

Species Reactivity N/A
UniProt N/A
Abbreviation OFX
Alternative Names N/A
Range 0.15-12.15 ppb
Sensitivity 0.15 ppb
Sample Type Tissue, aquatic, honey, egg, etc.
Detection Method Competitive ELISA
Analysis Method Quantitive
Assay Duration 1-3h
Sample Volume 10-200 μL
Detection Wavelengt 450 nm

Test principle

This assay is based on the competitive enzyme immunoassay for the detection of OFX in the sample. The coupling antigens are pre-coated on the micro-well stripes. The OFX in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-OFX antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the OFX in it. This value is compared to the standard curve and the OFX concentration is subsequently obtained.
 

Product Overview


 

Components


Reagents

Quantity

Reagents

Quantity

Assay plate (96 Wells)

1

Instruction manual

1

Standards

6 x 1 mL

Redissolving Solution (concentrate 2 x)

2 x 20 mL

Antibody

1 x 6 mL

HRP-Conjugate

1 x 6 mL

Wash Buffer (concentrate 20 x)

1 x 20 mL

Stop Solution

1 x 6 mL

Substrate A

1 x 6 mL

Substrate B

1 x 6 mL

Adhesive Films

4

 

Specificity


 

Recovery


Sample Type

Number

Recovery range (%)

Average(%)

Serum

10

90-101

96

EDTA plasma

10

89-97

93

Heparin plasma

10

91-99

95

 

Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
 

Linearity


Sample Type

1:2

1:4

1:8

1:16

Serum

78-89%

81-99%

92-103%

95-105%

EDTA plasma

91-101%

90-98%

93-101%

91-98%

Heparin plasma

92-103%

93-102%

92-99%

91-101%

 

Stability

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
 

Sample collection and storage

Store at 2-8°C. Please refer to Instruction Manual.
 

Kits storage instructions

Store at 2-8°C. Please refer to Instruction Manual.