Rabbit Anti-Human Dok-6 Polyclonal Antibody

DOK6 shares 75% and 78% identity with Dok4 and Dok5, respectively, over the PH and PTB domains and also shares a C-terminal region motif that may distinguish this subclass of DOK proteins. RT-PCR analysis of human tissues detected highest expression in the CNS, especially in fetal brain, and high expression in kidney and testis, as well as several Ret-positive neuroblastoma cell lines. In situ hybridization of mouse embryos detected Dok6 expression in the developing neocortex, diencephalon, and spinal cord, as well as in uteric buds of the developing kidney, and in the cranial parasympathetic, sensory, and sympathetic ganglia and the facial motor nucleus. The distribution of Dok6 developmental expression overlapped with Ret expression.

ELISA=1:2000-10000; WB=1:500-2000; IHC=1:100-300; IF=1:200-1000. Not yet tested in other applications. 

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.

Shipped at 2-8°C, Store it under sterile conditions at -20°C to -80°C upon receiving. **Avoid repeated freeze-thaw cycles.**