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Rabbit Anti-Rat Gastrin Polyclonal Antibody

Citations(0)Uniprot : Polyclonal antibody preparation

  • Cat.No.: PA91368RA

  • Reactivity: Rat (Rattus norvegicus)

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Product Details

Species Reactivity Rat (Rattus norvegicus)
Source Polyclonal antibody preparation
Host Rabbit
Synonyms GT; GAST; GAS; Gastrin component I; Big gastrin; Gastrin component II; Gastrin component III.
Concentration 1 mg/mL
Form Lyophilized or Liquid
Purity > 90% as determined by SDS-PAGE
Molecular Mass 12 kDa
Ig Type IgG
Purification Rabbit Polyclonal IgG, the antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Label None
Immunogen The antiserum was produced against synthesized peptide derived from rat Gastrin. AA range:52-101 (P04563).
Applications WB; IHC; IF; ELISA

Product Overview

Gastrin is a major physiological regulator of gastric acid secretion. It also has an important trophic or growth-promoting influence on the gastric mucosa. Gastrin is synthesized in G cells, which are located in gastric pits, primarily in the antrum region of the stomach and binds receptors found predominantly on parietal and enterochromaffin-like cells.In humans, gastrin is a hormone that stimulates secretion of gastric acid by the parietal cells of the stomach.
It is released by G cells in the stomach and duodenum. Gastrin is a linear peptide hormone produced by G cells of the duodenum and in the pyloric antrum of the stomach. In the Zollinger-Ellison syndrome, gastrin is produced at excessive levels, often by a gastrinoma of the antrum or the pancreas.

 

Specifity

Gastrin Polyclonal Antibody detects endogenous levels of Gastrin protein.
 

Usage

WB=1:500-2000 ELISA=1:2000-10000 IHC=1:100-300 IF=1:200-1000
Not yet tested in other applications. 
Optimal working dilutions must be determined by the end user.

 

Stability

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.
 

Storage

Shipped at 2-8°C, Store it under sterile conditions at -20°C to -80°C upon receiving.
**Avoid repeated freeze-thaw cycles.**

 

Stroage Buffer

0.01M PBS (pH 7.4) with 0.5% BSA, 0.02% sodium azide and 50% Glycerol.
 

Precautions

This product is for research use only, not for diagnostic or therapeutic use.
 

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