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Human Microalbunminuria (MAU/ALB) ELISA Kit

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  • Cat.No.: AE64710HU

  • Reactivity: Human (Homo sapiens)

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Product Details

Species Reactivity Human (Homo sapiens)
UniProt N/A
Abbreviation MAU/ALB
Alternative Names DKFZp779N1935; PRO0883; PRO0903; PRO1341; cell growth inhibiting protein 42|growth-inhibiting protein 20|serum albumin
Range Request Information
Sensitivity Request Information
Sample Type Serum, Plasma, Urine, Other biological fluids
Detection Method Sandwich
Analysis Method Quantitive
Assay Duration 1-4.5h
Sample Volume 1-200 μL
Detection Wavelengt 450 nm

Test principle

This assay employs a two-site sandwich ELISA to quantitate MAU/ALB in samples. An antibody specific for MAU/ALB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAU/ALB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAU/ALB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAU/ALB bound in the initial step. The color development is stopped and the intensity of the color is measured.

 

Product Overview


 

Components


Reagents

Quantity

Reagents

Quantity

Assay plate (96 Wells)

1

Instruction manual

1

Standard (lyophilized)

2

Sample Diluent

1 x 20 mL

Biotin-Conjugate (concentrate 100 x)

1 x 120 μL

Biotin-Conjugate Diluent

1 x 20 mL

Streptavidin-HRP  (concentrate 100 x)

1 x 120 μL

Streptavidin-HRP Diluent   

1 x 20 mL

Wash Buffer (concentrate 25 x)

1 x 20 mL

Substrate Solution

1 x 10 mL

Stop Solution

1 x 10 mL

Adhesive Films

4

 

Specificity

This assay has high sensitivity and excellent specificity for detection of Human MAU/ALB. No significant cross-reactivity or interference between Human MAU/ALB and analogues was observed.

 

Recovery

Matrices listed below were spiked with certain level of recombinant Human MAU/ALB and the recovery rates were calculated by comparing the measured value to the expected amount of Human MAU/ALB in samples.

Sample Type

Number

Recovery range (%)

Average(%)

Serum

10

90-101

96

EDTA plasma

10

89-97

93

Heparin plasma

10

91-99

95

 

Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
 

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human MAU/ALB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample Type

1：2

1：4

1：8

1：16

Serum

78-89%

81-99%

92-103%

95-105%

EDTA plasma

91-101%

90-98%

93-101%

91-98%

Heparin plasma

92-103%

93-102%

92-99%

91-101%

 

Stability

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
 

Sample collection and storage

Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 2 - 8°C before centrifugation for 15 minutes at 1000 × g. Remove serum and assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 × g at 2 - 8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles.
Urine: Collect urine using a metabolic cage. Remove any particulates by centrifugation for 15 minutes at 1000x g, 2-8°C and assay immediately or aliquot and store samples at -20°C or -80°C .Avoid repeated freeze-thaw cycles. Centrifuge again before assaying to remove any additional precipitates that may appear after storage. 
Other biological fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.

 

Kits storage instructions

Store at 2-8°C. Please refer to Instruction Manual.
 

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